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Background and purpose:This study aimed to estimate the spike-effect of domestic nanometer activated carbon on the radical operation for early stage low-lying rectal cancer. Methods:From Jan. 2013 to Dec. 2015, 66 patients with early stage low-lying rectal cancer were randomly divided into two groups:study group and control group. The patients of study group were treated with injection of carbon nanoparticles suspension in tumor vicinity before the operation. This study compared the total number of lymph node, the scale percentage of micro lymph node between two groups. SLNs of study group were obtained for pathological examination. Results:The differences in the total number of lymph node and the scale percentage of micro lymph node between two groups were statistically signiifcant (P<0.05). The diagnostic sensitivity and false-negative rate were 90.9%and 3.8%, respectively. Conclusion:Local injection of nanometer activated carbon around the tumor is important to the metastasis lymph node resection, especially to SLN biopsy in the radical operation for rectal cancer.
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Objective To study the association between metastatic ratio of lymph node and clinical pathological features and its prognosis in elderly patients with gastric cancer.Methods The first postoperative pathological data of 219 patients with gastric cancer aged ≥ 60 years were retrospectively analyzed.The relationships of metastatic ratio of lymph node with gender,differentiation degree,depth of tumor invasion,vascular invasion,perineural invasion were analyzed.We used Kaplan-Meier survival analysis and Cox regression model to analyze this relationship.Results The metastasis rate of lymph node was higher in gastric cancer patients with higher degree of differentiation,deeper tumor-infiltration,vascular invasion or neural invasion,and the metastasis rate of lymph node was increased in females than in males (all P＜0.05).Kaplan-Meier survival analysis and Cox regression model analysis suggested that the metastasis rate of lymph node was an independent factor for postoperative survival rate in the elderly with gastric cancer.Conclusions The metastatic ratio of lymph node could be used to estimate the clinical prognosis of elderly gastric cancer.
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Objective To explore the effect of different concentrations of ALLN on proliferation and apoptosis of C2C12 myoblasts.Methods After intervention with Ca2+ and ALLN,methyl thiazolyl tetrazolium and flow cytometry were used to determine the effect of Ca2+ and ALLN on the proliferation and apoptosis of C2C12 cells,respectively.The morphological changes of C2C12 myoblasts were observed using Giemsa staining.Results The absorbance of Ca2 + group was significantly lower than that of the control group (P ＜0.05).After 6,12,24,36 hours of intervention,the absorbance in ALLN groups 1 to 7 (cultured in serum-free media containing 16 mmol/L Ca2+ and ALLN at final concentrations of 3.125,6.25,12.5,25,50,100,200 μmol/L) were all significantly higher than that in the 16 mmol/L Ca2+ group (after 6 hours:0.449±0.024,0.472±0.022,0.513 ±0.008,0.540±0.014,0.588±0.016,0.607±0.030,0.700±0.020 vs.0.355 ±0.012,all P =0.000; after 12 hours:0.407 ±0.007,0.414 ±0.006,0.434 ±0.004,0.441 ±0.003,0.460 ±0.010,0.484 ± 0.006,0.525 ± 0.006 vs.0.368 ± 0.027,all P =0.000; after 24 hours:0.436±0.005,0.431 ±0.015,0.441 ±0.006,0.459 ±0.013,0.527 ±0.009,0.581 ±0.005,0.599 ±0.011 vs.0.386 ± 0.007,all P =0.000 ; after 36 hours:0.464 ± 0.022,0.460 ± 0.018,0.461 ± 0.007,0.434 ± 0.020,0.454 ± 0.028,0.479 ± 0.006,0.524 ± 0.011 vs.0.379 ± 0.011,all P =0.000),while no significant differences were observed after 48-72 hours of intervention.After treatment for 36 hours,the apoptosis rate in ALLN 10,50,100,and 200 μmol/L groups were (6.00 ± 1.20) ％,(5.02 ± 1.13) ％,(4.89±1.11)％,and (2.71 ± 1.15)％,all significantly lower than that in the Ca2+ group [(13.70 ±2.30)％] (all P =0.000).Giemsa staining showed apoptotic morphological changes in the Ca2+ group,which were obviously alleviated in the ALLN group.Conclusions Ca2+ at a concentration of 16mmol/L can induce apoptosis of C2C12 cells.In contrast,ALLN can inhibit cell apoptosis and promote proliferation in a time-and dose-dependent manner.
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Objective: To observe the effect of the combinative supplementation of Formoterol, Indomethacin and Roxithromycin on cancer cachexia (CC) and to study the mechanism. Methods: Male BALB/c mice bearing colon 26 adenocarcinoma for 9 days were served as models of cancer cachexia. The mice were divided into nine groups. Physiological conditions, body weight and food intake were documented every day. Serum levels of cytokine and nutritional markers were detected 7 days after treatment. Results: Tumor-bearing caused a wasting of non-tumor body weight and gastrocnemius muscle. Serum tumor necrosis factor-α, (TNF-α) and interleukin-6 (IL-6) levels were significantly elevated accompanied with nutrition depletion in tumor-bearing mice. After treatment with combinative supplementation of Formoterol, Indomethacin and Roxithromycin, left gastrocnemius weight and serum glucose were improved and the levels of TNF-α and triglyceride were down-regulated. Conclusion: Formoterol, Indomethacin and Roxithromycin had their own positive effects to CC mice. Drugs combinationin in tumor-bearing mice with cancer cachexia could produce synergistic action.
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Objective: To investigate the laboratory,clinical and economic results of early postoperative enteral nutrition(EEN) in patients with gastrointestinal cancer.Methods: 120 patients with gastrointestinal cancer were randomly divided into three groups: EEN,early postoperative parenteral nutrition(PN), and traditional nutrition(CON)groups.The 1/3 and 2/3 target volumes were administered on the first and second postoperative days(1POD,2POD),and the full volume was given from 3POD to 8POD in EEN and PN groups.The routine formula was offerd from 1POD to 8POD in CON group.The nutritional and immune parameters were detected one day before surgery and 9POD, and the clinical and economic results were collected in three groups.Results: The levels of ALB,TF,PA,CD3,CD4,CD4/CD8 were significantly higher in EEN and PN groups than those in CON group(both P＜0.01),and the CD3,CD4,CD4/CD8 were better in EEN group than those in PN group(P＜0.05).The side effects of EEN group were similar to those of PN group(P>0.05). The recovery of bowel function was quicker in EEN group than that in PN and CON groups(both P＜0.05).The complication rate, cost of nutrition support,and cost of complication treatment were lower in EEN group than those in PN group(P＜0.05, P＜0.01, P＜0.01).Although the cost of nutrition support was lowest but the complication rate and cost of complication treatment were all highest in CON group.Conclusion: EEN is better in immune function, recovery of bowel function,complication rate, and cost-effectiveness than those in PN, and from our multiple angular viewpoints EEN is the first choice for patients with gastrointestinal cancer.
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Objective:To evaluate the effect of Arg and Gln enriched PN on nutrition status,immune function and stress status of gastric cancer patients after radical treatment.Methods:Eighty patients with gastric cancer were randomly divided into 4 groups: arg group(arginine enriched PN group),Gln group(glntamine supplemented PN group),Arg+Gln group(arginine and glutamine enriched PN group)and Rou group(Routine PN group).The distinct immune nutrients were added into PN in the different groups for 7 consecutive days.The difference of the patients' nutrition status,immune function and stress status were analyzed after operation.Results: The nutritional station and immunue fuction in Arg group,Gln group and Arg+Gln group were better than in Rou group on the 8th postoperative day.The concentration of CRP in the Arg+Gln group was different from that in other groups(P
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Objective To explore the clinical effect of postoperative early enteral nutrition in the elderly patients with total gastrectomy. Methods Fifty seven elderly patients with total gastrectomy were divided into postoperative routine nutrition(PRN), postoperative early parenteral nutrition(PEPN), and postoperative early enteral nutrition(PEEN) groups. The levels of serum albumin(ALB), transferrin(TF), pre-albumin(PA), CD3, CD4, CD8, CD4/CD8 were detected 1 day before(PRED 1 ) and 9 days after(POD 9 ) operation, and the postoperative infectious complications were observed , and the length of hospital stay(LOS) and the cost of nutritional support were determined. Results The levels of ALB, TF, PA, CD3, CD4 and CD4/CD8 on POD 9 were (34.3?3.5)g/L, (1.7?0.2)g/L, (258.8?20.2)mg/L,(39.7?5.4)%,(34.3?4.0)% and (1.5?0.3) in PEPN as well as (33.9?3.1)g/L, (1.7?0.2)g/L, (260.5?24.7)mg/L, (42.8?6.5)%, (37.2?4.5)% and (1.7?0.2) respectively, which were all significantly lower than (29.5?3.0)g/L, (1.3?0.2)g/L, (235.4?13.9)mg/L, (34.8?5.0)%, (30.4?4.4)% and (1.2?0.2) in RN group (all P
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Objectives:To investigate the therapeutic effect of somatostatin and parenteral nutrition on early postoperative inflammatory intestinal obstruction. Methods:16 patients(test group) were treated with somatostatin and parenteral nutrition.Somatostatin was administered in 6 mg doses daily through continuous intravenous drip and the formula of parenteral nutrition was provided according to clinical routines.The therapeutic effect was compared with that of previous 13 cases(control group) without use of somatostatin and parenteral nutrition. Results:The rates of transferring to operation in the test group and the control group were 12.5 % and 61.5%( P
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Objectives:To investigate the effect of GH added early postoperative enteral nutrition on protein metabolism and immune function. Methods:Thirty six patients undergoing total or subtotal gatrectomy were randomly divided into three groups:traditional entertal nutrition (TEN),early postoperative enteral nutrition(EPEN),and GH added early postoperative enteral nutrition(GH+EPEN).Nutritional support lasted for 7 days.GH daily dose was 8 IU.Nutritional markers and immune markers were detected in several time points. Results:On postoperative day 4,the levels of Alb,Tf,TCL,and CD4/CD8 were all significantly increased in GH+EPEN as compared with those of TEN(all P
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Objectives:To investigate the effect of growth hormone(GH) and parenteral nutrition (PN) on protein metabolism in patients with cirrhosis of liver. Methods:The test group received GH and PN,and the control group received PN only.All the treatments were maintained for 7 days.The serum levels of Alb,TFN and PA were detected on the day before the treatment and the day 3,5 and 7 after the treatment. Results:As compared with the day before the treatment,the levels of Alb,TFN and PA on the day 3,5 and 7 in the two groups were all increased.However,the changes in the test group were greater than those in the control group.As compared with the control group,the level of Alb on the day 7 and the levels of TFN and PA on the day 5 and 7 in the test group were significantly higher. Conclusions:The therapeutic value of combined use of GH and PN is much better than that of single PN in patients with cirrhosis of liver.
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Objective:To observe the effect of the combinative supplementation of Formoterol, Indomethacin and Roxithromycin on cancer cachexia (CC) and to study the mechanism. Methods: Male BALB/c mice bearing colon 26 adenocarcinoma for 9 days were served as models of cancer cachexia. The mice were divided into nine groups. Physiological conditions, body weight and food intake were documented every day. Serum levels of cytokine and nutritional markers were detected 7 days after treatment. Results: Tumor-bearing caused a wasting of non-tumor body weight and gastrocnemius muscle. Serum tumor necrosis factor-?, (TNF-?) and interleukin-6 (IL-6) levels were significantly elevated accompanied with nutrition depletion in tumor-bearing mice. After treatment with combinative supplementation of Formoterol, Indomethacin and Roxithromycin, left gastrocnemius weight and serum glucose were improved and the levels of TNF-? and triglyceride were down-regulated. Conclusion: Formoterol, Indomethacin and Roxithromycin had their own positive effects to CC mice. Drugs combinationin in tumor-bearing mice with cancer cachexia could produce synergistic action.[